Rhinovirus infection of the airway epithelium enhances mast cell immune responses via epithelial-derived interferons.

ABSTRACT:

Background

Mast cells (MCs) within the airway epithelium in asthma are closely related to airway dysfunction, but cross talk between airway epithelial cells (AECs) and MCs in asthma remains incompletely understood. Human rhinovirus (RV) infections are key triggers for asthma progression, and AECs from individuals with asthma may have dysregulated antiviral responses.

Objective

We utilized primary AECs in an ex vivo coculture model system to examine cross talk between AECs and MCs after epithelial rhinovirus infection.

Methods

Primary AECs were obtained from 11 children with asthma and 10 healthy children, differentiated at air-liquid interface, and cultured in the presence of laboratory of allergic diseases 2 (LAD2) MCs. AECs were infected with rhinovirus serogroup A 

SUBMITTER: Murphy RC 

PROVIDER: S-EPMC10257743 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature