Trap and ambush therapy using sequential primary and tumor escape-selective oncolytic viruses.

ABSTRACT: In multiple models of oncolytic virotherapy, it is common to see an early anti-tumor response followed by recurrence. We have previously shown that frontline treatment with oncolytic VSV-IFN-β induces APOBEC proteins, promoting the selection of specific mutations that allow tumor escape. Of these mutations in B16 melanoma escape (ESC) cells, a C-T point mutation in the cold shock domain-containing E1 (CSDE1) gene was present at the highest frequency, which could be used to ambush ESC cells by vaccination with the mutant CSDE1 expressed within the virus. Here, we show that the evolution of viral ESC tumor cells harboring the escape-promoting CSDE1^C-T mutation can also be exploited by a virological ambush. By sequential delivery of two oncolytic VSVs in viv

SUBMITTER: Webb MJ 

PROVIDER: S-EPMC10258242 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature