Screening of Exons 4-9 Polymorphisms of FTO Gene in Endometrial and Ovarian Cancers.

ABSTRACT: Endometrial and ovarian tumours are almost mechanistically affected by reproductive hormones. Ovarian cancer may be explained as metastatic or synchronous primary ovarian cancer, and the specific diagnosis is a challenge. This study aimed to investigate the mutations in fat mass and obesity-associated (FTO) genes and investigated the association of these mutations with the risk of endometrial and ovarian cancers as well as with cancer grade and stage. Blood samples were collected from 48 endometrial and ovarian cancer cases and 48 healthy women. Genomic DNA was extracted, and PCR was done to amplify FTO exons 4-9. Sanger sequencing identified 6 different novel mutations submitted to DDBJ: p.W278G and p.G284G in exon 4, p.S318I and p.A324G in exon 5 and two mutations in intron

SUBMITTER: Khudheyer Almyah M 

PROVIDER: S-EPMC10258247 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature