Generation of sheep with defined FecB^B and TBXT mutations and porcine blastocysts with KCNJ5^G151R/+ mutation using prime editing.

ABSTRACT:

Background

Rewriting the genomes of living organisms has been a long-standing aim in the biological sciences. The revelation of the CRISPR/Cas9 technology has revolutionized the entire biological field. Since its emergence, this technology has been widely applied to induce gene knockouts, insertions, deletions, and base substitutions. However, the classical version of this system was imperfect for inducing or correcting desired mutations. A subsequent development generated more advanced classes, including cytosine and adenine base editors, which can be used to achieve single nucleotide substitutions. Nevertheless, these advanced systems still suffer from several limitations, such as the inability to edit loci without a suitable PAM sequence and to induce base transversions. On the

SUBMITTER: Zhou S 

PROVIDER: S-EPMC10258939 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature