Characterization of aberrant glycosylation associated with osteoarthritis based on integrated glycomics methods.

ABSTRACT:

Background

Osteoarthritis (OA) is the most common form of arthritis, affecting millions of aging people. Investigation of abnormal glycosylation is essential for the understanding of pathological mechanisms of OA.

Methods

The total protein was isolated from OA (n = 13) and control (n = 11) cartilages. Subsequently, glycosylation alterations of glycoproteins in OA cartilage were investigated by lectin microarrays and intact glycopeptides analysis. Finally, the expression of glycosyltransferases involved in the synthesis of altered glycosylation was assessed by qPCR and GEO database.

Results

Our findings revealed that several glycopatterns, such as α-1,3/6 fucosylation and high-mannose type of N-glycans were altered in OA cartilages. Notably, over 27% of identified glyc

SUBMITTER: Yu H 

PROVIDER: S-EPMC10258941 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature