ROS accumulation-induced tapetal PCD timing changes leads to microspore abortion in cotton CMS lines.

ABSTRACT:

Background

Cytoplasmic male sterility (CMS) is the basis of heterosis exploitation. CMS has been used to hybrid production in cotton, but its molecular mechanism remains unclear. CMS is associated with advanced or delayed tapetal programmed cell death (PCD), and reactive oxygen species (ROS) may mediate this process. In this study, we obtained Jin A and Yamian A, two CMS lines with different cytoplasmic sources.

Results

Compared with maintainer Jin B, Jin A anthers showed advanced tapetal PCD with DNA fragmentation, producing excessive ROS which accumulated around the cell membrane, intercellular space and mitochondrial membrane. The activities of peroxidase (POD) and catalase (CAT) enzymes which can scavenge ROS were significantly decreased. However, Yamian A tapetal PCD wa

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10259065 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature