Minimal Kidney Disease Phenotype in Shroom3 Heterozygous Null Mice.

ABSTRACT:

Background

Shroom family member 3 (SHROOM3) encodes an actin-associated protein that regulates epithelial morphology during development. Several genome-wide association studies (GWAS) have identified genetic variances primarily in the 5' region of SHROOM3, associated with chronic kidney disease (CKD) and poor transplant outcomes. These genetic variants are associated with alterations in Shroom3 expression.

Objective

Characterize the phenotypic abnormalities associated with reduced Shroom3 expression in postnatal day 3-, 1-month and 3-month-old mice.

Methods

The Shroom3 protein expression pattern was determined by immunofluorescence. We generated Shroom3 heterozygous null mice (Shroom3^Gt/+) and performed comparative analyses with wild

SUBMITTER: Lawlor A 

PROVIDER: S-EPMC10259099 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature