Precise temporal control of neuroblast migration through combined regulation and feedback of a Wnt receptor.

ABSTRACT: Many developmental processes depend on precise temporal control of gene expression. We have previously established a theoretical framework for regulatory strategies that can govern such high temporal precision, but experimental validation of these predictions was still lacking. Here, we use the time-dependent expression of a Wnt receptor that controls neuroblast migration in Caenorhabditis elegans as a tractable system to study a robust, cell-intrinsic timing mechanism in vivo. Single-molecule mRNA quantification showed that the expression of the receptor increases non-linearly, a dynamic that is predicted to enhance timing precision over an unregulated, linear increase in timekeeper abundance. We show that this upregulation depends on transcriptional activation, providing in vivo e

SUBMITTER: Schild ES 

PROVIDER: S-EPMC10259474 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature