High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography.

ABSTRACT: X-ray crystallography is the most commonly employed technique to discern macromolecular structures, but the crucial step of crystallizing a protein into an ordered lattice amenable to diffraction remains challenging. The crystallization of biomolecules is largely experimentally defined, and this process can be labor-intensive and prohibitive to researchers at resource-limited institutions. At the National High-Throughput Crystallization (HTX) Center, highly reproducible methods have been implemented to facilitate crystal growth, including an automated high-throughput 1,536-well microbatch-under-oil plate setup designed to sample a wide breadth of crystallization parameters. Plates are monitored using state-of-the-art imaging modalities over the course of 6 weeks to provide insight into cry

SUBMITTER: Budziszewski GR 

PROVIDER: S-EPMC10259656 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature