N6-methyladenosine modification of CENPF mRNA facilitates gastric cancer metastasis via regulating FAK nuclear export.

ABSTRACT:

Background

N6-methyladenosine (m⁶ A) modification is the most common modification that occurs in eukaryotes. Although substantial effort has been made in the prevention and treatment of gastric cancer (GC) in recent years, the prognosis of GC patients remains unsatisfactory. The regulatory mechanism between m⁶ A modification and GC development needs to be elucidated. In this study, we examined m⁶ A modification and the downstream mechanism in GC.

Methods

Dot blotting assays, The Cancer Genome Atlas analysis, and quantitative real-time PCR (qRT-PCR) were used to measure the m⁶ A levels in GC tissues. Methylated RNA-immunoprecipitation sequencing and RNA sequencing were performed to identify the targets of m⁶ A modificatio

SUBMITTER: Xu P 

PROVIDER: S-EPMC10259669 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature