Palladium encapsulated mesoporous silica nanoparticles for the rapid detection of analytes.

ABSTRACT: We designed a simple, inexpensive, and user-friendly assay using mesoporous silica nanoparticles to detect analytes. Highly stable and uniform palladium nanoparticles covered with mesoporous silica (Pd@mSiO₂) were generated and characterized extensively using physical methods. Human Serum Albumin (HSA) protein or ssDNA specific to the HIV gag region was capped onto the Pd@mSiO₂ electrostatically. This "cap" prevented the Pd(0) inside the mesoporous silica nanoparticles from catalyzing the conversion of non-fluorescent molecules to fluorescent molecules. In the presence of target anti-HSA antibodies or complementary sequence (HIV gag), HSA protein or DNA cap dissociated from the surface of Pd@mSiO₂-NH₂ through the specific antigen-antibody reactio

SUBMITTER: Jia T 

PROVIDER: S-EPMC10259872 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature