An unusual tandem kinase fusion protein confers leaf rust resistance in wheat.

ABSTRACT: The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with disease-resistance genes¹. Here we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata². We established that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Aegilops triuncialis³, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotyp

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10260399 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature