Dynamics of Sequence and Structural Cell-Free DNA Landscapes in Small-Cell Lung Cancer.

ABSTRACT:

Purpose

Patients with small-cell lung cancer (SCLC) have an exceptionally poor prognosis, calling for improved real-time noninvasive biomarkers of therapeutic response.

Experimental design

We performed targeted error-correction sequencing on 171 serial plasmas and matched white blood cell (WBC) DNA from 33 patients with metastatic SCLC who received treatment with chemotherapy (n = 16) or immunotherapy-containing (n = 17) regimens. Tumor-derived sequence alterations and plasma aneuploidy were evaluated serially and combined to assess changes in total cell-free tumor load (cfTL). Longitudinal dynamic changes in cfTL were monitored to determine circulating cell-free tumor DNA (ctDNA) molecular response during therapy.

Results

Combined tiered analyses of tumor-derived seq

SUBMITTER: Sivapalan L 

PROVIDER: S-EPMC10261918 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature