A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila.

ABSTRACT: Chemical neurotransmission occurs at specialized contacts where neurotransmitter release machinery apposes neurotransmitter receptors to underlie circuit function. A series of complex events underlies pre- and postsynaptic protein recruitment to neuronal connections. To better study synaptic development in individual neurons, we need cell-type-specific strategies to visualize endogenous synaptic proteins. Although presynaptic strategies exist, postsynaptic proteins remain less studied because of a paucity of cell-type-specific reagents. To study excitatory postsynapses with cell-type specificity, we engineered dlg1[4K], a conditionally labeled marker of Drosophila excitatory postsynaptic densities. With binary expression systems, dlg1[4K] labels central and peripheral

SUBMITTER: Parisi MJ 

PROVIDER: S-EPMC10261928 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature