Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium.

ABSTRACT:

Background

Nudibranchs comprise a group of > 6000 marine soft-bodied mollusk species known to use secondary metabolites (natural products) for chemical defense. The full diversity of these metabolites and whether symbiotic microbes are responsible for their synthesis remains unexplored. Another issue in searching for undiscovered natural products is that computational analysis of genomes of uncultured microbes can result in detection of novel biosynthetic gene clusters; however, their in vivo functionality is not guaranteed which limits further exploration of their pharmaceutical or industrial potential. To overcome these challenges, we used a fluorescent pantetheine probe, which produces a fluorescent CoA-analog employed in biosynthesis of secondary metabolites, to label and captu

SUBMITTER: Dzunkova M 

PROVIDER: S-EPMC10262491 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature