Evolution of carbapenem resistance in klebsiella pneumoniae and escherichia coli carrying bla_NDM-1 gene: imipenem exposure results in sustained resistance memory of strains in vitro.

ABSTRACT:

Background

Antibiotics exert an outstanding selective pressure on bacteria, forcing their chromosomal gene mutations and drug resistance genes to spread. The objective of this study is to evaluate the expression of the New Delhi Metallo-β-Lactamase-1 gene (bla_NDM-1) in the clinical isolate (Klebsiella pneumoniae TH-P12158), transformant strains Escherichia coli BL21 (DE3)-bla_NDM-1, and Escherichia coli DH5α- bla_NDM-1 when exposed to imipenem.

Methods

β-Lactamase genes (bla_SHV, bla_TEM-1, bla_CTX-M-9, bla_IMP, bla_NDM-1, bla_KPC, bla_OXA, bla_GES, and bla_DHA) from randomly selected carbapenems-sensitive K.pneumoniae (n = 20) and E.coli (n = 20) strains

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC10262507 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature