Systematic approaches to C-lignin engineering in Medicago truncatula.

ABSTRACT:

Background

C-lignin is a homopolymer of caffeyl alcohol present in the seed coats of a variety of plant species including vanilla orchid, various cacti, and the ornamental plant Cleome hassleriana. Because of its unique chemical and physical properties, there is considerable interest in engineering C-lignin into the cell walls of bioenergy crops as a high-value co-product of bioprocessing. We have used information from a transcriptomic analysis of developing C. hassleriana seed coats to suggest strategies for engineering C-lignin in a heterologous system, using hairy roots of the model legume Medicago truncatula.

Results

We systematically tested strategies for C-lignin engineering using a combination of gene overexpression and RNAi-mediated knockdown in the caffeic acid/5-hy

SUBMITTER: Ha CM 

PROVIDER: S-EPMC10262568 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature