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Eating while intoxicated: characterizing the molecular mechanism behind V. cholerae toxin MakA-regulated autophagy.

ABSTRACT: Extracellular pathogens utilize secreted virulence factors to regulate host cell function. Recently we characterized the molecular mechanism behind host macroautophagy/autophagy regulation by the Vibrio cholerae toxin MakA. Cholesterol binding at the plasma membrane induces MakA endocytosis and pH-dependent pore assembly. Membrane perforation of late endosomal membranes induces cellular membrane repair pathways and V-ATPase-dependent unconventional LC3 lipidation on damaged membranes.

SUBMITTER: Corkery DP

PROVIDER: S-EPMC10262753 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Eating while intoxicated: characterizing the molecular mechanism behind V. cholerae toxin MakA-regulated autophagy.

Corkery Dale P DP Wu Yao-Wen YW

Autophagy 20221121 6

Extracellular pathogens utilize secreted virulence factors to regulate host cell function. Recently we characterized the molecular mechanism behind host macroautophagy/autophagy regulation by the Vibrio cholerae toxin MakA. Cholesterol binding at the plasma membrane induces MakA endocytosis and pH-dependent pore assembly. Membrane perforation of late endosomal membranes induces cellular membrane repair pathways and V-ATPase-dependent unconventional LC3 lipidation on damaged membranes. ...[more]

PMID: 36409136

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