PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS.

ABSTRACT: Peroxisomes are rapidly degraded during amino acid and oxygen deprivation by a type of selective autophagy called pexophagy. However, how damaged peroxisomes are detected and removed from the cell is poorly understood. Recent studies suggest that the peroxisomal matrix protein import machinery may serve double duty as a quality control machinery, where they are directly involved in activating pexophagy. Here, we explored whether any matrix import factors are required to prevent pexophagy, such that their loss designates peroxisomes for degradation. Using gene editing and quantitative fluorescence microscopy on culture cells and a zebrafish model system, we found that PEX13, a component of the peroxisomal matrix import system, is required to prevent the degradation of otherwise healthy pero

SUBMITTER: Demers ND 

PROVIDER: S-EPMC10262789 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature