Biological condensates form percolated networks with molecular motion properties distinctly different from dilute solutions.

ABSTRACT: Formation of membraneless organelles or biological condensates via phase separation and related processes hugely expands the cellular organelle repertoire. Biological condensates are dense and viscoelastic soft matters instead of canonical dilute solutions. To date, numerous different biological condensates have been discovered, but mechanistic understanding of biological condensates remains scarce. In this study, we developed an adaptive single-molecule imaging method that allows simultaneous tracking of individual molecules and their motion trajectories in both condensed and dilute phases of various biological condensates. The method enables quantitative measurements of concentrations, phase boundary, motion behavior, and speed of molecules in both condensed and dilute phases, as well as

SUBMITTER: Shen Z 

PROVIDER: S-EPMC10264073 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature