Single-nucleus multi-omics of human stem cell-derived islets identifies deficiencies in lineage specification.

ABSTRACT: Insulin-producing β cells created from human pluripotent stem cells have potential as a therapy for insulin-dependent diabetes, but human pluripotent stem cell-derived islets (SC-islets) still differ from their in vivo counterparts. To better understand the state of cell types within SC-islets and identify lineage specification deficiencies, we used single-nucleus multi-omic sequencing to analyse chromatin accessibility and transcriptional profiles of SC-islets and primary human islets. Here we provide an analysis that enabled the derivation of gene lists and activity for identifying each SC-islet cell type compared with primary islets. Within SC-islets, we found that the difference between β cells and awry enterochromaffin-like cells is a gradient of cell states rather than a stark differ

SUBMITTER: Augsornworawat P 

PROVIDER: S-EPMC10264244 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature