ScChIX-seq infers dynamic relationships between histone modifications in single cells.

ABSTRACT: Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression. Recent advances have enabled mapping of histone marks in single cells, but most methods are constrained to profile only one histone mark per cell. Here, we present an integrated experimental and computational framework, scChIX-seq (single-cell chromatin immunocleavage and unmixing sequencing), to map several histone marks in single cells. scChIX-seq multiplexes two histone marks together in single cells, then computationally deconvolves the signal using training data from respective histone mark profiles. This framework learns the cell-type-specific correlation structure between histone marks, and therefore does not require a priori assumptions of their genomic

SUBMITTER: Yeung J 

PROVIDER: S-EPMC10264247 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature