Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems.

ABSTRACT: Around 50% of humankind relies on groundwater as a source of drinking water. Here we investigate the age, geochemistry, and microbiology of 138 groundwater samples from 95 monitoring wells (<250 m depth) located in 14 aquifers in Canada. The geochemistry and microbiology show consistent trends suggesting large-scale aerobic and anaerobic hydrogen, methane, nitrogen, and sulfur cycling carried out by diverse microbial communities. Older groundwaters, especially in aquifers with organic carbon-rich strata, contain on average more cells (up to 1.4 × 10⁷ mL^-1) than younger groundwaters, challenging current estimates of subsurface cell abundances. We observe substantial concentrations of dissolved oxygen (0.52 ± 0.12 mg L^-1 [mean ± SE]; n = 57) in older groundwa

SUBMITTER: Ruff SE 

PROVIDER: S-EPMC10264387 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature