Cell type-specific mapping of ion distribution in Arabidopsis thaliana roots.

ABSTRACT: Cell type-specific mapping of element distribution is critical to fully understand how roots partition nutrients and toxic elements with aboveground parts. In this study, we developed a method that combines fluorescence-activated cell sorting (FACS) with inductively coupled plasma mass spectrometry (ICP-MS) to assess the ionome of different cell populations within Arabidopsis thaliana roots. The method reveals that most elements exhibit a radial concentration gradient increasing from the rhizodermis to inner cell layers, and detected previously unknown ionomic changes resulting from perturbed xylem loading processes. With this approach, we also identify a strong accumulation of manganese in trichoblasts of iron-deficient roots. We demonstrate that confining manganese sequestration in trich

SUBMITTER: Giehl RFH 

PROVIDER: S-EPMC10264424 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature