Tuning the Microenvironment to Create Functionally Distinct Mesenchymal Stromal Cell Spheroids.

ABSTRACT: Mesenchymal stromal cells (MSCs) are under investigation for wound healing and tissue regeneration due to their potent secretome. Compared to monodisperse cells, MSC spheroids exhibit increased cell survival and enhanced secretion of endogenous factors such as vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE₂), two key factors in wound repair. We previously upregulated the proangiogenic potential of homotypic MSC spheroids by manipulating microenvironmental culture conditions. However, this approach depends on the responsiveness of host endothelial cells (ECs)-a limitation when attempting to restore large tissue deficits and for patients with chronic wounds in which ECs are dysfunctional and unresponsive. To address this challenge, we used a Design of Experime

SUBMITTER: Thai VL 

PROVIDER: S-EPMC10264490 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature