Comparison of gut microbiome composition in colonic biopsies, endoscopically-collected and at-home-collected stool samples.

ABSTRACT:

Aim

The goal of this study is to compare microbiome composition in three different sample types in women, namely stool brought from home vs. solid stool samples obtained at the time of an unprepped sigmoidoscopy vs. biopsies of the colonic mucosa at the time of an unprepped sigmoidoscopy, using alpha- and beta-diversity metrics following bacterial 16S rRNA sequencing. The findings may have relevance to health and disease states in which bacterial metabolism has a significant impact on molecules/metabolites that are recirculated between the gut lumen and mucosa and systemic circulation, such as estrogens (as in breast cancer) or bile acids.

Methods

Concomitant at-home-collected stool, endoscopically-collected stool, and colonic biopsy samples were collected from 48 subjects (

SUBMITTER: Nowicki C 

PROVIDER: S-EPMC10264612 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature