Stepwise protein targeting into plastoglobules are facilitated by three hydrophobic regions of rice phytoene synthase 2.

ABSTRACT: Plastoglobules (PGs) are plastidial lipid droplets enclosed by a polar monolayer born from the thylakoid membrane when plants require active lipid metabolism, including carotenogenesis, under the environmental stress and during plastid transition. Despite the fact that many proteins are reported to target PGs, their translocation mechanism has remained largely unexplored. To elucidate this process, we studied the influence of three hydrophobic regions (HR)-HR1 (1-45^th aa), HR2 (46-80^th aa), and HR3 (229-247^th aa)-of rice phytoene synthase 2 (OsPSY2, 398 aa), which has previously shown to target PGs. As results, HR1 includes the crucial sequence (31-45^th aa) for chloroplast import and the stromal cleavage occurs at a specific alanine site (64

SUBMITTER: Yu JS 

PROVIDER: S-EPMC10264786 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature