A p21-GFP zebrafish model of senescence for rapid testing of senolytics in vivo.

ABSTRACT: Senescence drives the onset and severity of multiple ageing-associated diseases and frailty. As a result, there has been an increased interest in mechanistic studies and in the search for compounds targeting senescent cells, known as senolytics. Mammalian models are commonly used to test senolytics and generate functional and toxicity data at the level of organs and systems, yet this is expensive and time consuming. Zebrafish share high homology in genes associated with human ageing and disease. They can be genetically modified relatively easily. In larvae, most organs develop within 5 days of fertilisation and are transparent, which allows tracking of fluorescent cells in vivo in real time, testing drug off-target toxicity and assessment of cellular and phenotypic changes. Here, we have g

SUBMITTER: Morsli S 

PROVIDER: S-EPMC10265157 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature