Different culture media and purification methods unveil the core proteome of Propionibacterium freudenreichii-derived extracellular vesicles.

ABSTRACT: Bacterial extracellular vesicles (EVs) are natural lipidic nanoparticles implicated in intercellular communication. Although EV research focused mainly on pathogens, the interest in probiotic-derived EVs is now rising. One example is Propionibacterium freudenreichii, which produces EVs with anti-inflammatory effects on human epithelial cells. Our previous study with P. freudenreichii showed that EVs purified by size exclusion chromatography (SEC) displayed variations in protein content according to bacterial growth conditions. Considering these content variations, we hypothesized that a comparative proteomic analysis of EVs recovered in different conditions would elucidate whether a representative vesicular proteome existed, possibly providing a robust proteome dataset for fu

SUBMITTER: Rodovalho VR 

PROVIDER: S-EPMC10265600 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature