Multivariate modular metabolic engineering for enhanced L-methionine biosynthesis in Escherichia coli.

ABSTRACT:

Background

L-Methionine is the only bulk amino acid that has not been industrially produced by the fermentation method. Due to highly complex and strictly regulated biosynthesis, the development of microbial strains for high-level L-methionine production has remained challenging in recent years.

Results

By strengthening the L-methionine terminal synthetic module via site-directed mutation of L-homoserine O-succinyltransferase (MetA) and overexpression of metA^fbr, metC, and yjeH, L-methionine production was increased to 1.93 g/L in shake flask fermentation. Deletion of the pykA and pykF genes further improved L-methionine production to 2.51 g/L in shake flask fermentation. Computer simulation and auxotrophic experiments verified that during the synthesis of L-methi

SUBMITTER: Li Z 

PROVIDER: S-EPMC10265765 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature