Transcriptional activation by LR1 at the Emu enhancer and switch region sites.

ABSTRACT: LR1 is a B cell-specific, sequence-specific duplex DNA binding activity which is induced in B cells carrying out class switch recombination. Here we identify several properties of LR1 which enable it to function in transcriptional regulation. We show that LR1 contributes to transcriptional activation by the Emu immunoglobulin heavy chain intron enhancer by binding to a site within the enhancer core. We further show that LR1 bends DNA upon binding. In addition, we show that LR1 is itself a bona fide transcriptional activator, as multimerized LR1 sites produce an element which can enhance transcription from a minimal promoter. In order for class switch recombination to occur, an activating signal must be transmitted via the Emu core, and both S regions targeted for recombination must be acti

SUBMITTER: Hanakahi LA 

PROVIDER: S-EPMC102658 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature