Calcium dynamics at the neural cell primary cilium regulate Hedgehog signaling-dependent neurogenesis in the embryonic neural tube.

ABSTRACT: The balance between neural stem cell proliferation and neuronal differentiation is paramount for the appropriate development of the nervous system. Sonic hedgehog (Shh) is known to sequentially promote cell proliferation and specification of neuronal phenotypes, but the signaling mechanisms responsible for the developmental switch from mitogenic to neurogenic have remained unclear. Here, we show that Shh enhances Ca²⁺ activity at the neural cell primary cilium of developing Xenopus laevis embryos through Ca²⁺ influx via transient receptor potential cation channel subfamily C member 3 (TRPC3) and release from intracellular stores in a developmental stage-dependent manner. This ciliary Ca²⁺ activity in turn antagonizes canonical, proliferative Shh sign

SUBMITTER: Shim S 

PROVIDER: S-EPMC10266006 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature