Melatonin regulates microglial polarization to M2 cell via RhoA/ROCK signaling pathway in epilepsy.

ABSTRACT:

Background

Melatonin (MEL), an endogenous hormone, has been widely investigated in neurological diseases. Microglia (MG), a resident immunocyte localizing in central nervous system is reported to play important functions in the animal model of temporal lobe epilepsy (TLE). Some evidence showed that MEL influenced activation of MG, but the detailed model of action that MEL plays in remains uncertain.

Methods

In this study, we established a model of TLE in mice by stereotactic injection of kainic acid (KA). We treated the mice with MEL. Lipopolysaccharide, ROCK2-knockdown (ROCK-KD) and -overexpression (ROCK-OE) of lentivirus-treated cells were used in cell experiments to simulate an in vitro inflammatory model.

Results

The results of electrophysiological tests showed th

SUBMITTER: Li P 

PROVIDER: S-EPMC10266134 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature