Transcriptome profiling of the initial segment and proximal caput of mouse epididymis.

ABSTRACT:

Background

The proximal region of the mouse epididymis plays a pivotal role in sperm transport, sperm maturation, and male fertility. Several studies have focused on segment-dependent gene expression of the mouse epididymis through high-throughput sequencing without the precision of the microdissection.

Methods and results

Herein, we isolated the initial segment (IS) and proximal caput (P-caput) by physical microdissection using an Lcn9-cre; Rosa26^tdTomato mouse model. We defined the transcriptome changes of caput epididymis by RNA sequencing (RNA-seq), which identified 1,961 genes that were abundantly expressed in the IS and 1,739 genes that were prominently expressed in the P-caput. In addition, we found that many differentially expressed ge

SUBMITTER: Wang X 

PROVIDER: S-EPMC10266198 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature