A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803.

ABSTRACT: Cyanobacteria are a promising platform for the production of the triterpene squalene (C30), a precursor for all plant and animal sterols, and a highly attractive intermediate towards triterpenoids, a large group of secondary plant metabolites. Synechocystis sp. PCC 6803 natively produces squalene from CO₂ through the MEP pathway. Based on the predictions of a constraint-based metabolic model, we took a systematic overexpression approach to quantify native Synechocystis gene's impact on squalene production in a squalene-hopene cyclase gene knock-out strain (Δshc). Our in silico analysis revealed an increased flux through the Calvin-Benson-Bassham cycle in the Δshc mutant compared to the wildtype, including the pentose phosphate pathway, as well

SUBMITTER: Germann AT 

PROVIDER: S-EPMC10266222 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature