HIV-1 activates oxidative phosphorylation in infected CD4 T cells in a human tonsil explant model.

ABSTRACT:

Introduction

Human immunodeficiency virus type 1 (HIV-1) causes a chronic, incurable infection leading to immune activation and chronic inflammation in people with HIV-1 (PWH), even with virologic suppression on antiretroviral therapy (ART). The role of lymphoid structures as reservoirs for viral latency and immune activation has been implicated in chronic inflammation mechanisms. Still, the specific transcriptomic changes induced by HIV-1 infection in different cell types within lymphoid tissue remain unexplored.

Methods

In this study, we utilized human tonsil explants from healthy human donors and infected them with HIV-1 ex vivo. We performed single-cell RNA sequencing (scRNA-seq) to analyze the cell types represented in the tissue and to investigate the impact of

SUBMITTER: Freeman TL 

PROVIDER: S-EPMC10266353 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature