Horizontal proton transfer across the antiporter-like subunits in mitochondrial respiratory complex I.

ABSTRACT: Respiratory complex I is a redox-driven proton pump contributing to about 40% of total proton motive force required for mitochondrial ATP generation. Recent high-resolution cryo-EM structural data revealed the positions of several water molecules in the membrane domain of the large enzyme complex. However, it remains unclear how protons flow in the membrane-bound antiporter-like subunits of complex I. Here, we performed multiscale computer simulations on high-resolution structural data to model explicit proton transfer processes in the ND2 subunit of complex I. Our results show protons can travel the entire width of antiporter-like subunits, including at the subunit-subunit interface, parallel to the membrane. We identify a previously unrecognized role of conserved tyrosine residues in cat

SUBMITTER: Zdorevskyi O 

PROVIDER: S-EPMC10266447 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature