Single-cell RNA-seq uncovers distinct pathways and genes in endothelial cells during atherosclerosis progression.

ABSTRACT: Background: Atherosclerosis (AS) is a chronic inflammatory disease involving various cell types, cytokines, and adhesion molecules. Herein, we aimed to uncover its key molecular mechanisms by single-cell RNA-seq (scRNA-seq) analysis. Methods: ScRNA-seq data of cells from atherosclerotic human coronary arteries were analyzed using the Seurat package. Cell types were clustered, and differentially expressed genes (DEGs) were screened. GSVA (Gene Set Variation Analysis) scores of hub pathways were compared among different cell clusters. DEGs in endothelial cells between apolipoprotein-E (ApoE)^-/- mice and specific TGFbR1/2 KO ApoE^-/- mice fed with high-fat diet were overlapped with those from human AS coronary arteries. In fluid shear stress and AS, hub gene

SUBMITTER: Wu M 

PROVIDER: S-EPMC10266549 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature