Adaptive Optical Two-Photon Fluorescence Microscopy Probes Cellular Organization of Ocular Lenses In Vivo.

ABSTRACT:

Purpose

The mammalian ocular lens is an avascular multicellular organ that grows continuously throughout life. Traditionally, its cellular organization is investigated using dissected lenses, which eliminates in vivo environmental and structural support. Therefore, in vivo optical imaging methods for studying lenses in their native context in live animals are urgently needed.

Methods

Here, we demonstrated that two-photon fluorescence microscopy can visualize lens cells in vivo. To maintain subcellular resolution at depth, we used adaptive optics to correct aberrations owing to ocular and lens tissues, which led to substantial signal and resolution improvements.

Results

Imaging lens cells up to 980 µm deep, we observed novel cellular organizations including suture-asso

SUBMITTER: Paidi SK 

PROVIDER: S-EPMC10266554 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature