Targeted IS-element sequencing uncovers transposition dynamics during selective pressure in enterococci.

ABSTRACT: Insertion sequences (IS) are simple transposons implicated in the genome evolution of diverse pathogenic bacterial species. Enterococci have emerged as important human intestinal pathogens with newly adapted virulence potential and antibiotic resistance. These genetic features arose in tandem with large-scale genome evolution mediated by mobile elements. Pathoadaptation in enterococci is thought to be mediated in part by the IS element IS256 through gene inactivation and recombination events. However, the regulation of IS256 and the mechanisms controlling its activation are not well understood. Here, we adapt an IS256-specfic deep sequencing method to describe how chronic lytic phage infection drives widespread diversification of IS256 in E. faecalis and how antibiotic exposure is associat

SUBMITTER: Kirsch JM 

PROVIDER: S-EPMC10266640 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature