Programming the Self-Organization of Endothelial Cells into Perfusable Microvasculature.

ABSTRACT: The construction of three-dimensional (3D) microvascular networks with defined structures remains challenging. Emerging bioprinting strategies provide a means of patterning endothelial cells (ECs) into the geometry of 3D microvascular networks, but the microenvironmental cues necessary to promote their self-organization into cohesive and perfusable microvessels are not well known. To this end, we reconstituted microvessel formation in vitro by patterning thin lines of closely packed ECs fully embedded within a 3D extracellular matrix (ECM) and observed how different microenvironmental parameters influenced EC behaviors and their self-organization into microvessels. We found that the inclusion of fibrillar matrices, such as collagen I, into the ECM positively influenced cell condensa

SUBMITTER: Cabral KA 

PROVIDER: S-EPMC10266707 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature