Establishment and characterization of oviductal organoids from farm and companion animals†.

ABSTRACT: Organoid technology has provided a unique opportunity to study early human development and decipher various steps involved in the pathogenesis of disease. The technology is already used in clinics to improve human patient outcomes. However, limited knowledge of the methodologies required to establish organoid culture systems in domestic animals has slowed the advancement and application of organoid technology in veterinary medicine. This is particularly true for the field of reproduction and the application of assisted reproductive technologies (ART). Here, we have developed a platform to grow oviductal organoids from five domestic species-bovine, porcine, equine, feline, and canine. The organoids were grown progressively from single cells derived from the enzymatic digestion of freshly co

SUBMITTER: Lawson EF 

PROVIDER: S-EPMC10266943 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature