Investigating the modulatory effects of Moringa oleifera on the gut microbiota of chicken model through metagenomic approach.

ABSTRACT:

Introduction

This study aimed to assess the effects of supplementing chicken feed with Moringa oleifera leaf powder, a phytobiotic, on the gastrointestinal microbiota. The objective was to examine the microbial changes induced by the supplementation.

Methods

A total of 40, one-day-old chickens were fed their basal diet for 42 days and then divided into two groups: SG1 (basal diet) and SG2 (basal diet + 10 g/kg Moringa oleifera leaf powder). Metagenomics analysis was conducted to analyze operational taxonomic units (OTUs), species annotation, and biodiversity. Additionally, 16S rRNA sequencing was performed for molecular characterization of isolated gut bacteria, identified as Enterococcus faecium. The isolated bacteria were tested for essential metabolit

SUBMITTER: Soundararajan S 

PROVIDER: S-EPMC10267347 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature