PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase.

ABSTRACT: Phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 5-phosphate (PI5P) are low-abundance phosphoinositides crucial for key cellular events such as endosomal trafficking and autophagy. Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) is an enzyme that regulates PI5P in vivo but can act on both PI5P and PI3P in vitro. In this study, we report a role for PIP4K in regulating PI3P levels in Drosophila Loss-of-function mutants of the only Drosophila PIP4K gene show reduced cell size in salivary glands. PI3P levels are elevated in dPIP4K ²⁹ and reverting PI3P levels back towards WT, without changes in PI5P levels, can rescue the reduced cell size. dPIP4K ²⁹ mutants also show up-regulation in autophagy and the reduced ce

SUBMITTER: Ghosh A 

PROVIDER: S-EPMC10267561 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature