A guide to Agrobacterium-mediated transformation of the chytrid fungus Spizellomyces punctatus.

ABSTRACT: Chytrid fungi play key ecological roles in aquatic ecosystems, and some species cause a devastating skin disease in frogs and salamanders. Additionally, chytrids occupy a unique phylogenetic position- sister to the well-studied Dikarya (the group including yeasts, sac fungi, and mushrooms) and related to animals- making chytrids useful for answering important evolutionary questions. Despite their importance, little is known about the basic cell biology of chytrids. A major barrier to understanding chytrid biology has been a lack of genetic tools with which to test molecular hypotheses. Medina and colleagues recently developed a protocol for Agrobacterium -mediated transformation of Spizellomyces punctatus. In this manuscript, we describe the general procedure including planni

SUBMITTER: Prostak SM 

PROVIDER: S-EPMC10267658 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature