Structures of human primosome elongation complexes.
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ABSTRACT:
SUBMITTER: He Q
PROVIDER: S-EPMC10268227 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Publications
Structures of human primosome elongation complexes.
He Qixiang Q Baranovskiy Andrey G AG Morstadt Lucia M LM Lisova Alisa E AE Babayeva Nigar D ND Lusk Benjamin L BL Lim Ci Ji CJ Tahirov Tahir H TH
Nature structural & molecular biology 20230417 5
The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA- ...[more]
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