Structures of human primosome elongation complexes.

ABSTRACT: The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers.

SUBMITTER: He Q 

PROVIDER: S-EPMC10268227 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature