Robust regulation of transcription pausing in <i>Escherichia coli</i> by the ubiquitous elongation factor NusG.

ABSTRACT: Transcription elongation by multi-subunit RNA polymerases (RNAPs) is regulated by auxiliary factors in all organisms. NusG/Spt5 is the only universally conserved transcription elongation factor shared by all domains of life. NusG is a component of antitermination complexes controlling ribosomal RNA operons, an essential antipausing factor, and a transcription-translation coupling factor in Escherichia coli. We employed RNET-seq for genome-wide mapping of RNAP pause sites in wild-type and NusG-depleted cells. We demonstrate that NusG is a major antipausing factor that suppresses thousands of backtracked and nonbacktracked pauses across the E. coli genome. The NusG-suppressed pauses were enriched immediately downstream from the translation start codon but were also abundant els

SUBMITTER: Yakhnin AV 

PROVIDER: S-EPMC10268239 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature