Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4^-/- mouse.

ABSTRACT:

Background

The primary pathological alterations of Pendred syndrome are endolymphatic pH acidification and luminal enlargement of the inner ear. However, the molecular contributions of specific cell types remain poorly characterized. Therefore, we aimed to identify pH regulators in pendrin-expressing cells that may contribute to the homeostasis of endolymph pH and define the cellular pathogenic mechanisms that contribute to the dysregulation of cochlear endolymph pH in Slc26a4^-/- mice.

Methods

We used single-cell RNA sequencing to identify both Slc26a4-expressing cells and Kcnj10-expressing cells in wild-type (WT, Slc26a4^+/+) and Slc26a4^-/- mice. Bioinformatic analysis of expression data confirmed marker genes defining the different cell type

SUBMITTER: Koh JY 

PROVIDER: S-EPMC10268361 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature