LncRNA5251 inhibits spermatogenesis via modification of cell-cell junctions.

ABSTRACT:

Background

Male factors-caused decline in total fertility has raised significant concern worldwide. LncRNAs have been identified to play various roles in biological systems, including spermatogenesis. This study aimed to explore the role of lncRNA5251 in mouse spermatogenesis.

Methods

The expression of lncRNA5251 was modulated in mouse testes in vivo or spermatogonial stem cells (C18-4 cells) in vitro by shRNA.

Results

The sperm motility in two generations mice after modulation of lncRNA5251 (muF0 and muF1) was decreased significantly after overexpression of lncRNA5251. GO enrichment analysis found that knockdown lncRNA5251 increased the expression of genes related to cell junctions, and genes important for spermatogenesis in mouse testes. Meanwhile, overexpressing ln

SUBMITTER: Zhang C 

PROVIDER: S-EPMC10268499 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature