Cryo-Electron Microscopy Structure of the Mycobacterium tuberculosis Cytochrome bcc:aa₃ Supercomplex and a Novel Inhibitor Targeting Subunit Cytochrome cI.

ABSTRACT: The mycobacterial cytochrome bcc:aa₃ complex deserves the name "supercomplex" since it combines three cytochrome oxidases-cytochrome bc, cytochrome c, and cytochrome aa₃-into one supramolecular machine and performs electron transfer for the reduction of oxygen to water and proton transport to generate the proton motive force for ATP synthesis. Thus, the bcc:aa₃ complex represents a valid drug target for Mycobacterium tuberculosis infections. The production and purification of an entire M. tuberculosis cytochrome bcc:aa₃ are fundamental for biochemical and structural characterization of this supercomplex, paving the way for new inhibitor targets and molecules. Here, we produced and purified the entire and

SUBMITTER: Mathiyazakan V 

PROVIDER: S-EPMC10269045 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature